A Pentaplex PCR Assay for the Detection and Differentiation of Shigella Species

Figure 2

Analytical sensitivity of multiplex PCR at genomic DNA level using reference strains. Lane 1, 100 bp plus marker; lane 2, positive control; lane 3, 100 ng/μL of genomic DNA S. flexneri; lane 4, 10 ng/μL of genomic DNA S. flexneri; lane 5, 1 ng/μL of genomic DNA S. flexneri; lane 6, 100 pg/μL of genomic DNA S. flexneri; lane 7, 10 pg/μL of genomic DNA S. flexneri; lane 8, 1 pg/μL of genomic DNA S. flexneri; lane 9, 100 bp plus marker; lane 10, positive control; lane 11, 100 ng/μL of genomic DNA S. sonnei; lane 12, 10 ng/μL of genomic DNA S. sonnei; lane 13, 1 ng/μL of genomic DNA S. sonnei; lane 14, 100 pg/μL of genomic DNA S. sonnei; lane 15, 10 pg/μL of genomic DNA S. sonnei; lane 16, 1 pg/μL of genomic DNA S. sonnei; lane 17, 100 bp plus marker; lane 18, positive control; lane 19, 100 ng/μL of genomic DNA S. dysenteriae; lane 20, 10 ng/μL of genomic DNA S. dysenteriae; lane 21, 1 ng/μL of genomic DNA S. dysenteriae; lane 22, 100 pg/μL of genomic DNA S. dysenteriae; lane 23, 10 pg/μL of genomic DNA S. dysenteriae; lane 24, 1 pg/μL of genomic DNA S. dysenteriae; lane 25, 100 bp plus marker.