BioMed Research International / 2013 / Article / Tab 1

Research Article

A Pentaplex PCR Assay for the Detection and Differentiation of Shigella Species

Table 1

Sequences of primers used for the pentaplex PCR.

Primers Primer sequence (5′-3′)Gene target Location
of gene
Amplicon size
(bp)
Target
identity
GenBank accession
number

SgenDF1TGC CCA GTT TCT TCA TAC GCinvC Plasmid875Shigella genus AF386526
SgenDR1GAA AGT AGC TCC CGA AAT GC
SflexDF1TTT ATG GCT TCT TTG TCG GCrfc Chromosome537Shigella flexneri CP000266
SflexDR1CTG CGT GAT CCG ACC ATG
SsonDF1TCT GAA TAT GCC CTC TAC GCTwbgZ Plasmid430Shigella sonnei CP000039
SsonDR1GAC AGA GCC CGA AGA ACC G
SdysDF1TCT CAA TAA TAG GGA ACA CAG CrfpB Plasmid211Shigella dysenteriae CP000640
SdysDR1CAT AAA TCA CCA GCA AGG TT
ICDF1GCA GGC ATT GCT GGG TAAompA Plasmid1319Internal control AY305875
ICDR1ACA CTT GTA AGT TTT CAA CTA CG