A Pentaplex PCR Assay for the Detection and Differentiation of Shigella Species

Table 1

Sequences of primers used for the pentaplex PCR.

Primers	Primer sequence (5′-3′)	Gene target	Location; of gene	Amplicon size; (bp)	Target; identity	GenBank accession; number
SgenDF1	TGC CCA GTT TCT TCA TAC GC	invC	Plasmid	875	Shigella genus	AF386526
SgenDR1	GAA AGT AGC TCC CGA AAT GC
SflexDF1	TTT ATG GCT TCT TTG TCG GC	rfc	Chromosome	537	Shigella flexneri	CP000266
SflexDR1	CTG CGT GAT CCG ACC ATG
SsonDF1	TCT GAA TAT GCC CTC TAC GCT	wbgZ	Plasmid	430	Shigella sonnei	CP000039
SsonDR1	GAC AGA GCC CGA AGA ACC G
SdysDF1	TCT CAA TAA TAG GGA ACA CAG C	rfpB	Plasmid	211	Shigella dysenteriae	CP000640
SdysDR1	CAT AAA TCA CCA GCA AGG TT
ICDF1	GCA GGC ATT GCT GGG TAA	ompA	Plasmid	1319	Internal control	AY305875
ICDR1	ACA CTT GTA AGT TTT CAA CTA CG