BioMed Research International / 2013 / Article / Tab 2

Research Article

A Pentaplex PCR Assay for the Detection and Differentiation of Shigella Species

Table 2

Bacterial species and strains used in this study and results of pentaplex PCR.

Bacterial strainsNo. of strains
inv rfc wbgZ rfpB IC (ompA)

S. flexneri (ATCC 12022)b1+++
S. sonnei (SH031)c1+++
S. boydii (ATCC 9207)b1++
S. dysenteriae (SD375)d1+++
Salmonella spp.2+
S. Typhic3+
S. Paratyphi 1+
S. Paratyphi Bc1+
Klebsiella spp.c2+
K. 2+
E. coli (EPEC)c1+
E. coli (EHEC)c1+
E. coli (ETEC)c1+
E. 4+
V. 3+
V. 1+
V. 1+
V. cholera (wild type)c1+
V. 1+
P. 3+
P. 1+
P. 1+
C. 1+
E. 1+
Y. 1+
Acinetobacter spp.c1+
A. 1+
S. 1+
Campylobacter spp.c1+
A. 1+
M. 1+
B. 1+
S. 2+
Methylene resistant S. 1+
Streptococcus spp. Group Ac1+
Streptococcus spp. Group Bc1+
Streptococcus spp. Group Gc1+
Corynebacterium spp.c1+
Listeria spp.c1+
Lactobacillus spp.c1+
Gardnerella spp.c1+

Shigella genus.
bReference strains from American Type Culture Collection (ATCC), Reston, VA, USA.
cDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia.
dObtained from Institute for Medical Research, Malaysia.
“+” is positive; “–” is negative by pentaplex PCR.