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BioMed Research International
Volume 2013 (2013), Article ID 412745, 6 pages
Research Article

The Study on Biocompatibility of Porous nHA/PLGA Composite Scaffolds for Tissue Engineering with Rabbit Chondrocytes In Vitro

1Guangzhou Medical College, Guangzhou, Guangdong 510182, China
2Department of Orthopedics, Second People’s Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China
3Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China
4Biomechanics and Medical Information Institute, Beijing University of Technology, Beijing 100022, China

Received 6 July 2013; Revised 4 September 2013; Accepted 4 September 2013

Academic Editor: Aaron W. James

Copyright © 2013 Lei Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering. Methods. Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry. Results. The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control ( ). Conclusion. The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.