BioMed Research International / 2013 / Article / Fig 4

Research Article

The Role of E3 Ubiquitin Ligase Cbl Proteins in Interleukin-2-Induced Jurkat T-Cell Activation

Figure 4

Jurkat T cells were exposed to IL-2 (250 U/mL) after using 25 μM PD98059 or depletion of ERK with small interfering RNA (siRNA). (a) after using 25 μM PD98059, p-ERK and ERK levels were measured by western blot. CD25 expression was measured by flow cytometry. (b) after depletion of ERK with small interfering RNA (siRNA), p-ERK and ERK levels were measured by western blot. CD25 expression was measured by flow cytometry. Mean ± S.E. ( ). *, .
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(a)
430861.fig.004b
(b)