BioMed Research International / 2013 / Article / Fig 5

Research Article

The Role of E3 Ubiquitin Ligase Cbl Proteins in Interleukin-2-Induced Jurkat T-Cell Activation

Figure 5

Effects of IL-2 (250 U/mL) on upregulating the expressions of c-Cbl and Cbl-b in Jurkat T cells. (a) The expression levels of Cbl-b and c-Cbl were measured by western blot in Jurkat T cells treated with 250 U/mL IL-2 for 48 h. (b) Jurkat T cells were exposed to IL-2 (250 U/mL) in the absence or presence of 10 nM PS341, and CD25 expression was measured by flow cytometry. (c) After exposure to 250 U/mL IL-2, the expressions of p-ERK, ERK, p-Akt, Akt, and tubulin were measured by western blot in the presence or absence of 10 nM PS341. (d) The intensity of p-ERK for IL-2 and/or PS341 treatment was estimated using NIH Image software as in (c) Mean ± SE ( ) from three separate experiments are presented.