Research Article

Acid Lipase from Candida viswanathii: Production, Biochemical Properties, and Potential Application

Table 2

Effect of culture pH on growth and lipase production by C. viswanathii.

Initial pHFinal pHBiomass (g/L)Specific activity (U/mg prot)Lipase activity (U) (g/g) (g/h) (U/g) (U/g·h)

3.0 1.0240.1583.6390.049
4.0 1.3100.2274.4960.049
5.0 1.2050.2255.9150.068
6.0 1.3120.2626.8070.073
7.0 1.3290.2052.3200.031
8.0 1.1270.2141.6180.019
9.0 1.4690.2081.9340.018
10.0 1.5770.2042.3640.020

Culture conditions: Vogel’s liquid medium with 1.5% (w/v) olive oil and 0.2% (w/v) yeast extract at 28°C, agitated at 210 rpm, for 72 h. : biomass yield on substrate consumed; : lipase yield on substrate consumed; : biomass productivity; : specific rate of lipase production.