Acid Lipase from Candida viswanathii: Production, Biochemical Properties, and Potential Application

Table 4

Effect of the surfactants and emulsifiers on growth and lipase production by C. viswanathii.

Emulsifiers (0.1%, w/v)	Lipase activity (U)	Specific activity (U/mg prot)	Biomass (g/L)	𝑌𝑋/𝑆 (g/g)	𝑃𝑋 (g/h)	𝑌𝐿/𝑆 (U/g)	𝑞𝐿 (U/g·h)
Control (no surfactant or emulsifier)	100.00±3.00	13.82±0.44	18.90±0.92	1.456	0.303	6.638	0.062
Tween 20	77.50±7.75	11.31±1.14	17.81±0.91	1.231	0.247	5.360	0.060
Tween 80	32.50±2.75	5.07±0.45	16.60±0.85	1.164	0.230	2.280	0.027
Triton X-100	5.75±0.50	0.89±0.08	5.55±0.62	0.776	0.077	0.804	0.014
Gum Arabic	65.00±5.25	1.53±0.16	16.83±0.30	1.408	0.233	5.439	0.055
Soybean lecithin	147.50±4.25	2.33±0.74	17.97±0.36	1.226	0.250	10.061	0.114
Sodium deoxycholate	52.50±4.25	0.52±0.04	14.11±1.04	1.033	0.196	3.849	0.051

Culture conditions: Vogel’s liquid medium with 1.5% (w/v) olive oil and 0.2% (w/v) yeast extract at 28°C, agitated at 210 rpm, pH 6.0, for 72 h. 𝑌_𝑋/𝑆: biomass yield on substrate consumed; 𝑌_𝐿/𝑆: lipase yield on substrate consumed; 𝑃_𝑋: biomass productivity; 𝑞_𝐿: specific rate of lipase production.