Acid Lipase from Candida viswanathii: Production, Biochemical Properties, and Potential Application

Table 5

Effect of organic solvents on lipase activity produced by C. viswanathii.

Organic solvents	Log P values	Relative activity (%)
		1%	10%
Control (no organic solvent added)		100±4.5	100±5.6
DMSO	−1.378	98.9±6.9	91.8±6.2
Methanol	−0.764	102.6±5.9	94.0±5.3
Acetonitrile	−0.394	89.8±7.1	16.8±5.2
Ethanol	−0.235	101.9±6.2	60.0±7.5
Acetone	−0.208	88.0±5.4	31.8±4.9
1-Propanol	0.250	109.1±9.1	18.6±9.7
Isopropanol	0.074	91.1±6.3	32.4±5.4
2-Propanol	0.070	88.1±6.5	29.2±9.5

Assay conditions: lipase activities were assayed on pNPP hydrolysis using McIlvaine buffer pH 3.5, at 40°C. Log P: logarithm of the partition coefficient (P) in octanol/water two-phase system indicates the solvents hydrophobicity. DMSO: dimethylsulfoxide.