Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2013, Article ID 451679, 11 pages
Research Article

Immunobiologic and Antiinflammatory Properties of a Bark Extract from Ampelozizyphus amazonicus Ducke

1Departamento de Imunologia, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, CCS Bloco I, 2° andar, 21941-590 Rio de Janeiro, RJ, Brazil
2Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, CCS Bloco K, 21941-590 Rio de Janeiro, RJ, Brazil
3Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, CCS Bloco A 2° andar, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brazil
4Universidade Federal do Rio de Janeiro, Instituto de Nutrição Josué de Castro, CCS Bloco JSS08, 21941-590 Rio de Janeiro, RJ, Brazil
5Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, CCS Bloco H, Rio de Janeiro, RJ, Brazil
6Università di Salerno, Dipartimento di Farmácia, Via Ponte Don Melillo, 84084 Fisciano, Italy

Received 7 October 2012; Revised 28 December 2012; Accepted 29 December 2012

Academic Editor: Fabio Ferreira Perazzo

Copyright © 2013 Ligia Maria Torres Peçanha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ampelozizyphus amazonicus is used in the treatment and prevention of malaria. The effect of an aqueous extract from this plant (SART) on the immune response was investigated by measuring immunoglobulin production induced by immunization with the antigen TNP-Ficoll in Plasmodium chabaudi-infected mice. SART treatment increased antigen-specific IgM and IgG levels in TNP-Ficoll-immunized mice. The B cell response during malarial infection was also modified by SART. There was an increase in total serum IgM and IgG and a decrease in the percentage of splenic plasma cells (CD138+ cells) in P. chabaudi-infected, SART-treated animals. SART (1, 3 or 10 mg/kg, p.o.) and the reference drug dexamethasone (5 mg/kg) were also tested in carrageenan-induced leukocyte migration to the subcutaneous air pouch (SAP). All SART doses significantly reduced leukocyte migration into the SAP. The protein concentration resulting from extravasation into the peritoneum was also significantly reduced. Our data indicate that SART possesses immunomodulatory properties, inducing an in vivo modification of the B lymphocyte response and anti-inflammatory properties, which are partly due to a reduction in cell migration and are most likely due to an inhibition of the production of inflammatory mediators. Preliminary HPLC-ESI-MS/MS analysis of SART shows a complex saponin profile with deprotonated molecule [M-H] ions in the range of m/z 800–1000.