Research Article

Chromogenic In Situ Hybridization and p16/Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6/E7 mRNA Test, and Potential Clinical Applications

Figure 1

CISH positive signals. Diffuse pattern, where nuclei are completely stained ((a), 20x magnification). Punctated pattern in invasive squamous cervical cancer: distinct dot-like intranuclear signals were noted within cells infiltrating the stroma ((b), 100x magnification). Mixed patterns, where both diffuse and punctated signals are noted ((c), 40x magnification).