Effects of Tamarindus indica Fruit Pulp Extract on Abundance of HepG2 Cell Lysate Proteins and Their Possible Consequential Impact on Metabolism and Inflammation

Table 2

List of cell lysate proteins of altered abundance in T. indica fruit extract-treated cells identified by MALDI-TOF/TOF MS/MS.

Spot no.	Protein description	Acc. no.	Score	pI/Mw (kDa)	Av. % FC^a	% Cov^b	Matched peptide; sequences	Functional; category
657	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 10 (CI-42 kD)	O95299	194	8.67/40.73	−1.8	44	117–122; 13–139; 140–161; 253–261; 290–295	Mitochondrial respiratory chain
765	Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial precursor core protein II	P22695	537	8.74/48.41	−1.5	28	71–84; 163–183; 184–196; 200–217; 232–241
900	NADH dehydrogenase (ubiquinone) flavoprotein 1, mitochondrial precursor (CI-51 kD)	P49821	275	8.51/50.79	−1.9	33	72–81; 153–159; 160–174; 376–386; 441–449
796	Ethanolamine-phosphate cytidylyltransferase	Q99447	339	6.44/43.81	−1.7	38	185–199; 262–271; 333–348	Phospholipid biosynthesis
590	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	P04406	192	8.57/36.03	−1.5	18	235–248; 310–323	Carbohydrate metabolism
722	GDP-L-fucose synthetase	Q13630	280	6.13/35.87	−1.5	49	26–44; 82–88; 90–107; 200–214; 291–297; 307–320
655	GMP reductase 2	Q9P2T1	386	6.79/37.85	−1.5	52	70–78; 178–189; 192–213; 277–286; 278–286; 292–298; 292–306	Nucleotide and nucleoside metabolism
914	Uridine 5′-monophosphate synthase (UMP synthase)	P11172	379	6.81/52.20	−1.6	37	6–22; 30–41; 146–155; 353–363; 389–405; 460–467; 469–477
363	S-methyl-5-thioadenosine phosphorylase (MTAP)	Q13126	253	6.75/31.23	−1.5	43	12–29; 72–82; 83–99; 100–116; 134–147; 181–187; 272–282	Polyamine metabolism
1634	Prohibitin	P35232	66	2.27/29.79	−1.7	21	134–143	Cell proliferation and differentiation
675	Eukaryotic translation initiation factor 3 subunit 3 (elF3H)	O15372	279	6.09/39.91	−1.5	43	52–75; 242–249; 260–265; 304–313; 314–331	Protein biosynthesis
751	Elongation factor Tu, mitochondrial precursor (EF-Tu)	P49411	114	7.26/49.51	−1.8	29	105–120; 239–252
1084	Tyrosyl-tRNA synthetase, cytoplasmic (TyrRS)	P54577	96	6.61/59.11	−1.5	21	85–93; 179–189; 433–450
540	Heterogenous nuclear ribonucleoprotein H3 (hnRNP H3)	P31942	409	6.37/36.90	−1.7	39	56–67; 85–90; 98–104; 206–222; 223–232; 262–287; 288–301	Others

aNegative value signifies down-regulation in terms of fold-differences. All ratios are statistically significant with 𝑃<0.05 (Student’s 𝑡-test).
^b% Coverage of the identified sequence.