Molecular Characterization and Clinical Impact of TMPRSS2-ERG Rearrangement on Prostate Cancer: Comparison between FISH and RT-PCR
Figure 1
(a) Patterns obtained in the tricolor probe FISH analysis. Three fused signals indicate two normal alleles (A1); a balanced translocation pattern is considered with a fused (RGB) signal and an adjacent BG with a separated R signals (A2); interstitial deletion is indicated by a RGB signal and a B and R adjacent signals (A3); one RGB signal plus only one R, B, or G signal (A4, A5, A6, resp.) corresponds to undetermined rearrangements. (b) Patterns of the dual color break-apart assay: normal pattern with two RG signals (B1); balanced translocation is indicated by a RG signal plus a G and R separated signals (B2); one RG signal plus a R signal indicates interstitial deletion (B3).
