Modulation of the Effects of Lung Immune Response on Bone Marrow by Oral Antigen Exposure
Figure 4
Effect of splenic T lymphocytes on bone marrow eosinopoiesis in a transfer protocol. T lymphocytes were isolated from spleens of BP-2 donor mice (either OVA/OVA/OVA tolerized or H2O/OVA/OVA controls) and used for i.v. transfers into syngeneic recipients. Recipient mice were either naïve and subsequently sensitized and challenged (T/OVA/OVA) or sensitized before transfer (OVA/T/OVA), challenge occurring after transfer. Data are Mean + SEM of EPO+ cell counts in 7-day IL-5 stimulated bone marrow cultures ((a), (c)) or freshly harvested bone marrow (b). (a) Eosinophil production ex vivo from donor (left) and recipient mice (right). T/OVA/OVA mice received T cells from control (black column) or tolerized (white column) donors. ; for the indicated differences; (b), (c) EPO+ cell counts in bone marrow (b) and eosinophil production ex vivo (c) in control H2O/OVA/OVA mice (black columns) or in recipients of T cells from OVA/OVA/OVA donors, with transfer before sensitization (T/OVA/OVA, white bars), after sensitization but before boost (OVA/T/OVA, light grey bars), or after sensitization and boost (OVA/T/OVA, dark grey bars). ; (b); (c) for the indicated differences.
