Licochalcone A-Induced Human Bladder Cancer T24 Cells Apoptosis Triggered by Mitochondria Dysfunction and Endoplasmic Reticulum Stress

Figure 3

LCA induced apoptosis in T24 cells. The cells were treated with or without the indicated amounts of LCA for 48 h with or without NAC (500 μM). (a) Morphologic measurements in T24 cells were carried out via Hoechst fluorescence staining. (b) Detection of apoptotic rates conducted via flow cytometry. (c) Bcl-2 and Bax expressions were analyzed via RT-PCR (upper), and quantitative analysis of Bcl-2 and Bax expressions mRNA levels (lower). (d) Bcl-2 and Bax expressions were analyzed via western blot (upper), and quantitative analysis of Bcl-2 and Bax protein expressions (lower). Control group (LCA-untreated group) level was accepted to be “1.0”. ^∗𝑃<0.05, ^∗∗𝑃<0.01 compared with the control group; ^#𝑃<0.05, ^##𝑃<0.01 compared with the LCA group alone (50 μM).

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