Figure 1: Training protocol. Schematic diagram representing the details of the experimental schedule in this study. Thirty-six Sprague-Dawley rats were used. The rats were divided into 2 groups: high-frequency electrical stimulation (HFS) and low-frequency electrical stimulation (LFS). Rats were subjected to training on every other day. (a) The left illustration represents 1 bout of exercise. (b) The right illustration represents 6 sessions of a training protocol. The rats in all of the experimental groups had their medial gastrocnemius muscle extracted after death.