|Table 2: NRAMP1 and VDR polymorphism sites and sequence variants.|
|Name||Nucleotide and amino acid change||Primers, 5′ to 3′||Polymorphic site||Genopytes||Fragment (bp)|
|Deletion of TGTG in the 3′UTR (55 nt 3′ to the last codon in exon 15) ||GCATCTCCCCAATTCATGGT|| ||TGTGdel/del||240|
|AACTGTCCCACTCTATCCTG||TGGA TGTGGA||TGTG+/del||240, 211, 33|
|(240 or 242 bp)||FokI||TGTG+/+||211, 33|
|VDR-FokI||A T/C transition|
polymorphism in exon 2
|ATGGAAACACCTTGCTTCTTCTCCCTC||CAGGGA TGGAGG||T/C-Ff||267, 197, 70|
|(267 bp)|| FokI||T/T-ff||197, 70|
|VDR-TaqI||A single base change ||CAGAGCATGGACAGGGAGC|| ||T/T-TT||455|
|C to T in codon 352 at the 3′ end of the||AGGAGAGGCAGCGGTACTG||ACAGGAG CTCT||T/C-Tt||455, 290, 165|
|VDR gene||(455 bp)||TaqI||C/C-tt||290, 165|
|Note: Underlined letters denote different restriction sites in which bold letters represent polymorphism sites. Genotypes of both genes were defined as follows. For the NRAMP1 gene, individuals were scored as 3′UTR-TGTG+/+ wild homozygote, 3′UTR-TGTGdel/del mutant homozygote, and 3′UTRTGTG+/del heterozygote, respectively.|
+= presence of TGTG; del = absence of these four bases.
For the VDR gene, individuals were scored as FokI-FF, TaqI-TT wild homozygotes, FokI-ff, TaqI-tt mutant homozygotes, FokI-Ff, and TaqI-Tt heterozygotes, respectively.