Identification of Protein Complex Associated with LYT1 of Trypanosoma cruzi
Analysis of EGFP and LYT1s+n-EGFP chimeric proteins expression. (a) Protein extracts of WT parasites and EGFP and LYT1s+n-EGFP transgenic parasites were run on polyacrylamide gels under denaturing conditions and transferred, and the exogenous proteins were revealed using an α-EGFP antibody. (b) Protein extracts of WT parasites and EGFP and LYT1s+n-EGFP transgenic parasites were dotted under native conditions and revealed using an α-EGFP or α-T. cruzi antibody. (c) Protein extract of WT parasites analyzed by SDS-PAGE and Coomassie-blue stain.