Affinity Labeling of Membrane Receptors Using Tissue-Penetrating Radiations

Figure 1

Affinity labeling of SDT with 50 uM of RTI-38 using UV or X-ray. Irreversible and specific binding of RTI-38 to SDT is noted with 90 cGy of X-ray or with UV (𝐹=840, 𝑃<0.001). t-tests and one-way ANOVA indicate statistically significant difference from the control group (𝑡=39.5, 𝑃=0.010) or the cocaine group treated with X-ray (𝑡=45.7, 𝑃=0.009).