Figure 5: Affinity labeling to PBDZR with PK14105 using UV, gamma rays, or ultrasounds. Affinity binding of PK14105 to PBDZR under UV, gamma rays, and ultrasounds is confirmed. Two-way ANOVA was used to analyze all data found significant for gamma rays doses ( and ) and PK14105 (with and ). Ultrasonic exposure (2 Watts/sq. cm at 1 MHz) of the reactants on ice in the dark for 2 and 10 minutes affects binding of 69% and 80%, respectively, in single-point experiments.