Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611

Table 5

Composition of various experiments of the central composite design (CCD) for independent variables and predicted and experimental values of responses.

Run no.	Sucrose (w/v)	Yeast extract (w/v)	NaNO3 (w/v)	Zn^+2 (v/v)	Triton X-100 (v/v)	Extracellular inulinase (U/mL)		Extracellular invertase (U/mL)		Biomass mg/mL		C/N
						Actual	Predicted	Actual	Predicted	Actual	Predicted	Actual	Predicted
1	16.00	4.00	1.00	2.00	0.50	1032.23	1140.22	1318.67	1311.74	24.25	24.51	2.1	2.09
2	16.00	1.00	3.00	2.00	0.50	1430.86	1367.56	1764.30	1698.08	36.29	37.83	1.5	1.56
3	4.00	4.00	3.00	1.00	1.50	219.53	123.44	458.12	461.95	19.00	16.24	2.6	2.65
4	16.00	4.00	3.00	1.00	0.50	862.46	867.01	903.05	833.45	21.18	19.06	2.5	2.51
5	16.00	4.00	1.00	1.00	1.50	884.57	748.36	1128.66	1059.05	20.21	20.19	2.4	2.45
6	16.00	1.00	1.00	2.00	1.50	1332.80	1297.23	1395.53	1416.14	34.61	34.69	1.7	1.63
7	4.00	1.00	3.00	2.00	1.50	1235.41	1172.11	1342.99	1293.56	29.04	30.75	2.0	2.02
8	4.00	4.00	1.00	2.00	1.50	1030.99	1038.33	1293.56	1376.84	23.20	23.00	2.2	2.19
9	16.00	1.00	3.00	1.00	1.50	1402.18	1366.60	1621.43	1625.26	35.40	34.74	1.6	1.61
10	4.00	4.00	3.00	2.00	0.50	930.16	970.30	1202.20	1198.66	22.44	21.60	2.3	2.38
11	4.00	1.00	1.00	1.00	0.50	1259.39	1163.32	1377.56	1321.19	29.76	29.64	1.9	1.96
12	4.00	2.50	2.00	1.50	1.00	1282.62	1490.61	1382.73	1404.97	30.81	33.02	1.8	1.60
13	16.00	2.50	2.00	1.50	1.00	1598.38	1756.51	2068.23	2256.15	37.11	38.01	1.4	1.35
14	10.00	1.00	2.00	1.50	1.00	2167.92	2461.75	2413.59	2561.17	39.37	36.80	1.1	1.02
15	10.00	4.00	2.00	1.50	1.00	1904.32	1976.61	2352.84	2415.42	38.69	44.36	1.2	1.03
16	10.00	2.50	1.00	1.50	1.00	2247.08	2399.61	2452.98	2481.99	39.62	39.62	1.0	0.98
17	10.00	2.50	4.00	1.50	1.00	1881.45	2095.04	2306.64	2487.78	38.07	41.18	1.3	1.07
18	10.00	2.50	2.00	1.00	1.00	2259.90	2619.30	2496.12	2684.04	40.96	46.63	0.9	0.73
19	10.00	2.50	2.00	2.00	1.00	3681.21	3687.93	3854.48	3876.72	73.39	70.82	0.1	0.02
20	10.00	2.50	2.00	1.50	0.50	2287.32	2294.04	2528.36	2731.03	43.17	44.44	0.8	0.60
21	10.00	2.50	2.00	1.50	1.50	2383.92	2743.32	2677.46	2684.94	43.29	45.13	0.7	0.64
22	10.00	2.50	2.00	1.50	1.00	2726.29	2681.88	2854.61	2887.25	49.57	48.29	0.6	0.61
23	10.00	2.50	2.00	1.50	1.00	2959.09	2681.88	3079.77	2887.25	48.69	48.29	0.4	0.61
24	10.00	2.50	2.00	1.50	1.00	3166.54	2681.88	3105.58	2887.25	52.16	48.29	0.3	0.61
25	10.00	2.50	2.00	1.50	1.00	2807.25	2681.88	2939.38	2887.25	49.92	48.29	0.5	0.61
26	10.00	2.50	2.00	1.50	1.00	3214.72	2681.88	3297.57	2887.25	53.55	48.29	0.2	0.61