Figure 3: Coimmunoprecipitation assays. (a) Coimmunoprecipitation of PIST and NaPi2a using rat kidney homogenates from rats fed a control diet, and with an anti-PIST antibody. (b) Coimmunoprecipitation in rats acutely adapted for 4 hours from low to high (L-H) and from high to low (H-L) Pi diet, using non-BBM homogenates. SN: supernatant (nonprecipitated samples); IP: immunoprecipitated; WB: Western blotting.