Figure 5: Effect of SAN on ROS levels in MCF-7 cells using a flow cytometry. 10000 MCF-7 cells were cultured into 6-well plates and were treated with indicated concentrations of SAN for 24 h. In the fluorescence intensity of NAC against ROS in MCF-7 cells which treated with SAN, the antioxidant NAC (10 mM) was also added together with the SAN for 24 h.