Figure 7: SAN decrease VEGF mRNA expression. MCF-7 cells were treated with SAN (with the indicated concentration) for 24 h. Total mRNA was extracted, and relative VEGF mRNA amounts were measured by real-time PCR. VEGF mRNA levels are expressed as fold change over the basal after normalizing to β-actin. VEGF mRNA levels were suppressed by 4 μM and 8 μM of SAN in MCF-7 cultures, and NAC and SOD can block the VEGF mRNA suppression. Each bar is the mean  ±  SD of three independent experiments. Statistical different from basal (vehicle-treated) was calculated by Dunnett’s test after ANOVA, ; compared with SAN treatment alone group, aa, bb mean .