Estrogen Signaling through Estrogen Receptor Beta and G-Protein-Coupled Estrogen Receptor 1 in Human Cerebral Vascular Endothelial Cells: Implications for Cerebral Aneurysms
Figure 1
Localization of ERβ and GPER in human cerebral vascular endothelial cells. (a) ERβ was immunocytochemically stained in red by a rabbit monoclonal antiestrogen receptor beta antibody and Alexa-594 conjugated secondary antibody at the nuclei of the cells. (b) GPER was immunocytochemically identified in red by anti-G-protein-coupled estrogen receptor antibody and Alexa-594 conjugated secondary antibody at the cytoplasm surrounding the nuclei of the cells when the cell membrane was permeabilized by Triton X-100. Low density of GPER was observed at the nuclei of the cells. (c) GPER signals were dispersed around the plasma membrane of the cells when the cells were not permeabilized. Immunocytochemistry, bar = 10 μm.
