Estrogen Signaling through Estrogen Receptor Beta and G-Protein-Coupled Estrogen Receptor 1 in Human Cerebral Vascular Endothelial Cells: Implications for Cerebral Aneurysms
The interaction events between ERβ and 17β-estradiol in human cerebral vascular endothelial cells. The interaction events between ERβ and 17β-estradiol were identified by in situ proximity ligation assay in the nuclei of the cells. The nuclei of the cells were positively stained by DAPI in blue. The interaction events between ERβ and 17β-estradiol were identified in red dots in the nuclei of the cells when the cells were exposed to the concentration of 17β-estradiol at 0 (a), 120 (b), 240 (c), 480 (d), 720 (e), or 960 pmol/L (f). In situ proximity ligation assay, bar = 10 μm.