Estrogen Signaling through Estrogen Receptor Beta and G-Protein-Coupled Estrogen Receptor 1 in Human Cerebral Vascular Endothelial Cells: Implications for Cerebral Aneurysms
Figure 3
The dose-response curve of interaction events between ERβ and 17β-estradiol in human cerebral vascular endothelial cells. When the cells were exposed to the increasing concentrations of 17β-estradiol from 120 to 960 pmol/L, the interaction events between ERβ and 17β-estradiol were increased (, ). At least 100 cells per concentration were quantified using ImageJ software. The positive PLA events were observed as red fluorescent particles (sizes from 2 to 50 pixels in diameter). When PLA events merged to create particles larger than 50 pixels, the area was measured, and number of events was assumed to be particle area divided by 10 since 10 pixels were the median size of most PLA events. Data were expressed as mean ± SEM of 3 separate experiments.
