Estrogen Signaling through Estrogen Receptor Beta and G-Protein-Coupled Estrogen Receptor 1 in Human Cerebral Vascular Endothelial Cells: Implications for Cerebral Aneurysms
Figure 5
Dose responsiveness of interaction events between GPER and 17β-estradiol in permeabilized human cerebral vascular endothelial cells. When the cells were exposed to different concentrations of 17β-estradiol ranging from 120 to 960 pmol/L, the interaction events between GPER and 17β-estradiol responded to the corresponding dose of 17β-estradiol. * versus the number of interaction signals at other concentrations of 17β-estradiol. At least 100 cells per condition were quantified using ImageJ software. The positive PLA signals were observed as red dots (sizes from 2 to 50 pixels in diameter). When PLA events merged to create particles larger than 50 pixels, the area was measured, and number of events was assumed to be particle area divided by 10 since 10 pixels were the median size of most PLA events. Data were expressed as mean ± SEM of 3 separate experiments.
