Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain

Figure 5

Multiple sequence alignment of Par25-like proteins from sand flies: P. arabicus (Ara), P. ariasi (Ari), P. perniciosus Italian strain (PerIta), and P. perniciosus Spanish strain (PerSpa), P. tobbi (Tob), P. duboscqi (Dub). Accession numbers are indicated in the sequence name. Sequences without signal peptide were aligned with ClustalW and refined using Boxshade server, and the percentage of the identities or similarities that must agree for shading was set at 80%. Black background shading represents identical amino acids, and grey shading designates similar amino acids, while white shading indicates no similarity. (Ø) indicates changes in the prediction of the secondary structure between P. perniciosus Spanish and Italian strains as performed by Protean (DNASTAR, Lasergene). (↓) signs above amino acids indicate changes in phosphorylation sites as predicted by NetPhos 2.0 Server, and the amino acid affected by the prediciton on the phosphorylation site is encircled.