Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain

Table 1

Polymorphisms between the current cDNAs sequenced from salivary glands of Phlebotomus perniciosus from Madrid and their corresponding best matches in NR database.

Protein name/NCBI accession number

Best match to NR nucleotide database

Protein family

BLASTn identities

Nucleotidic polymorphisms^a

Aminoacidic polymorphisms^a

Changes in glycosylation sites^c

Changes in phosphorylation sites^d

Changes in protein structure^e

Gaps

Identities

UTRs

CDS

Antigenic regions^b

Nonantigenic regions^b

N

O

SP01 (HE974344)

PperSP01 (DQ192490)

Apyrase

0/999 (0%)

982/999 (98%)

0

17

1

1

No

No

Yes

No

SP01B (HE974345)

PperSP01B (DQ192491)

Apyrase

16/1116 (1%)

1089/1116 (98%)

3

8

4

2

No

No

Yes

Yes

SP02 (HE985074)

PperSP02 (DQ150620)

PpSP15-like

0/522 (0%)

513/522 (98%)

3

6

2

1

No

No

Yes

No

SP02 (HE985075)

PperSP02 (DQ150620)

PpSP15-like

1/523 (0%)

513/523 (98%)

3

6

3^f

0^f

No

No

Yes

Yes

SP02 (HE985076)

PperSP02 (DQ150620)

PpSP15-like

2/524 (0%)

511/524 (98%)

2

9

5

1

No

No

Yes

No

SP02 (HE985077)

PperSP02 (DQ150620)

PpSP15-like

3/525 (0%)

511/525 (97%)

1

10

4

1

No

No

Yes

No

SP03B (HE974346)

PperSP03B (DQ150622)

Yellow protein

0/1182 (0%)

1165/1182 (99%)

0

17

4

4

No

No

Yes

Yes

SP04 (HE980444)

PperSP04 (DQ150623)

D7-related

0/838 (0%)

821/838 (98%)

5

12

4

1

No

No

No

No

SP04B (HE980443)

PperSP04B (DQ150624)

D7-related

2/824 (0%)

761/824 (92%)

9

52

22

5

No

No

Yes

Yes

SP06 (HE970770)

PperSP06 (DQ153100)

Lufaxin

1/1031 (0%)

1011/1031 (98%)

8

11

3

1

No

No

Yes

Yes

SP08 (HE974347)

PperSP08 (DQ153102)

ParSP25-like

0/774 (0%)

766/774 (99%)

0

8

3

1

No

No

Yes

Yes

SP09 (HE966456)

PperSP09 (DQ153103)

PpSP15-like

0/500 (0%)

477/500 (95%)

7

16

8

1

No

No

Yes

Yes

SP11 (HE974348)

PperSP11 (DQ153105)

PpSP15-like

10/483 (2%)

458/483 (95%)

11

4

1

0

No

No

No

No

Hypothetical protein P119 (HE985078)

Aedes aegypti hypothetical protein (XM_001663068)

Unknown

0/496 (0%)

402/496 (81%)

18

76

8

1

No

Yes

Yes

Yes

aNucleotidic and aminoacid polymorphisms between the current sequenced cDNA clones and their best P. perniciosus salivary cDNA matches (BLASTn and ClustalW for nucleotidic and amino acidic alignments).
The number of nucleotidic polymorphisms fits the values of nonidentical nucleotides (column 5) minus the gaps (column 4).
bProtein antigenic regions were predicted by in silico bioinformatics (Jameson-Wolf index, Protean program, DNAstar, Lasergene).
cN-, and O-glycosylation sites prediction performed with NetNGlyc 1.0 Server and NetOGlyc 3.1 Server.
dPhosphorylation sites prediction performed with NetPhos 2.0 Server.
eAlpha helix and beta sheet locations were predicted by Garnier-Robson algorithm (Protean program, DNASTAR, Lasergene).
fTwo deletions in the nucleotidic sequence of SP02 (HE985075) lead to a switch of the reading frame of the gene.