Figure 2: Efflux of nucleotides (material absorbing at 260 nm), K+, and phosphate produced by chitosan at variable concentrations on the strain C. albicans ATCC 10231. Cells (50 mg, wet weight) were incubated for 10 min at 30°C in 10 mM MES-TEA, pH 6.0, and 20 mM glucose, with (G + Sorb) or without (G) 0.8 M sorbitol, in a final volume of 4.0 mL and the indicated concentrations of chitosan. After incubation, the cells were centrifuged, and the different materials were measured in the supernatant as described under experimental procedures. (a) Material absorbing at 260 nm; (b) potassium, and (c) phosphate.