Effects of Chitosan on Candida albicans: Conditions for Its Antifungal Activity
Figure 6
Viability of C. albicans, strain ATCC 10231 after incubation with variable concentrations of chitosan under different conditions. Nonstarved cells (2.5 mg) and successive tenfold dilutions in sterile water were incubated in 96-well plastic plates as described under experimental procedures for 10 min at 30°C in 10 mM MES-TEA buffer, pH 6.0 plus (a) 20 mM glucose; (b) 20 mM glucose and 10 mM KCl; (c) YPD medium only; (d) 110 mM glucose; (e) 20 mM glucose and 2% bactopeptone; (f) 20 mM glucose and 1% yeast extract, and each one with variable amounts of chitosan in μg·mL−1 as indicated, in a final volume of 0.2 mL. Then, they were spotted using a solid pin multiplate replicator in solid YPD medium. Images were captured after 48 h of growth.
