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BioMed Research International
Volume 2013 (2013), Article ID 529589, 18 pages
Research Article

Recovery of Fertility in Azoospermia Rats after Injection of Adipose-Tissue-Derived Mesenchymal Stem Cells: The Sperm Generation

1Ankalife IVF and Women Health Centre, Ankara, Turkey
2Stem Cell Department, Institute of Health Sciences, Kocaeli University Center for Stem Cell and Gene Therapies Research and Practice, Izmit, 41380 Kocaeli, Turkey
3Urology Department, Faculty of Medicine, Ufuk University, Ankara, Turkey
4Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey
5Pathology Department, Ankara Hospital, Ankara, Turkey

Received 10 October 2012; Revised 6 December 2012; Accepted 9 December 2012

Academic Editor: Thomas Skutella

Copyright © 2013 Cihangir Cakici et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Immunostaining of undifferentiated rAT-MSCs for VASA and SCP1. The expression of meiotic (SCP1) and spermatogenic cell markers (VASA) was not observed in stem cell cultures in vitro. DAPI was used for nuclei staining (blue). Scale bars: 50µm.

Supplementary Figure 2: Immunostaining of rAT-MSCs for GFP. GFP labeled rAT-MSCs were stained with antibody to GFP (Santa Cruz, sc-5385) (A1-A3). The staining pattern of GFP+ MSCs was cytoplasmic, but most of the luminescence was observed around the nuclei. The GFP staining of rAT-MSCs were compared with the negative control, untransformed rAT-MSCs (B1-B3).

Supplementary Figure 3: GFP+ sperms from offspring. Sperms from offspring collected on glass slides by the cytocentrifuge were stained with GFP antibody (A1-A3). The nuclei were labeled with DAPI. The control slides were stained with secondary antibody and DAPI, to show the autofluorescence (B1-B3). Scale bars: 20µm.

  1. Supplementary Figure 1
  2. Supplementary Figure 2
  3. Supplementary Figure 3