Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
Figure 2
Morphology of cardiac cryoinjury model and engrafted engineered cardiomyocyte sheets. (a): (A) and (B) show cryoinjury on the epicardial surface of the left ventricle and transplantation with engineered cardiomyocyte sheets. (C) and (D) show representative hematoxylin/eosin staining of cross section of cryoinjured hearts without or with cardiomyocyte sheets transplantation 4 weeks after operation. Asterisk shows cardiomyocyte sheets transplanted on the epicardial surface of cryoinjured heart. (b): (A) shows representative hematoxylin/eosin staining of cardiomyocyte sheets 4 weeks after transplantation; (B)–(D) show the serial sections immunolabeling with -actinin (AA, red),which marks cardiomyocytes, and GFP (green), which marks grafts. The grafted cardiomyocyte sheets can be identified by double positive for AA and GFP. Scale bars: 2 mm in Figures 2(a)C and 2(a)D; 200 μm in Figure 2(b). AA: -actinin.
