Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
Figure 3
Plates with 2-FA resistant foci of wild type Physcomitrella (a and b) and pplig4 (c) after 3 weeks of selection. (a) Untreated Physcomitrella wild type, (b) 50 μg mL−1 Bleomycin treated wild type protonemata for 2 hours, and (c) 5 μg mL−1 Bleomycin treated pplig4 protonemata for 1 hour prior being spread on plates with BCDAT medium supplemented with 2 μM 2-FA and cultivated for 3 weeks.