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BioMed Research International
Volume 2013, Article ID 536814, 6 pages
Research Article

Coating with Autologous Plasma Improves Biocompatibility of Mesh Grafts In Vitro: Development Stage of a Surgical Innovation

1Department of Urology, Lukas Hospital, Preußenstraße 84, 41464 Neuss, Germany
2West German Cancer Center (WTZ), University of Essen, Essen, Germany
3German Centre for Assessment and Evaluation of Innovative Techniques in Medicine (DZITM), Germany
4Department of Surgery, Lukas Hospital, Neuss, Germany
5German Centre for Implant-Pathology, Düren, Germany
6Department of Experimental Surgery, University of Szeged, Hungary

Received 16 June 2013; Accepted 15 August 2013

Academic Editor: Thorsten Ecke

Copyright © 2013 Holger Gerullis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials. Materials and Methods. Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs), platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue assay, representative for fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. Adherence of those tissues on the meshes was microscopically investigated and semiquantitatively assessed using a previously described scoring system. Results. Coating with peripheral blood mononuclear cells did not affect the adherence score, whereas coating with platelets and blood plasma increased the score suggesting improved biocompatibility in vitro. The previous ranking of native meshes remained consistent after coating. Conclusion. Plasma coating of meshes improves their biocompatibility score in a novel in vitro test system.