A Phytase Characterized by Relatively High pH Tolerance and Thermostability from the Shiitake Mushroom Lentinus edodes

Table 1

Summary of purification procedure of L. edodes phytase (from 2000 g fresh fruiting bodies).

Purification step	Yield (mg)	Total activity (U)^a	Specific activity (U/mg)^b	Recovery rate (%)	Purification fold^c
Water extract	4515.0	406.4	0.09	100	1
DEAE cellulose	498.2	303.9	0.61	74.8	6.8
CM cellulose	252.3	219.5	0.87	54.0	9.7
Blue gel	137.8	154.3	1.12	38.0	12.4
Q-sepharose	63.5	112.4	1.77	27.7	19.7
Superdex 75	27.1	84.3	3.11	20.7	34.6

^aTotal activity: phytase activity (U/mL) in each step × volume (mL);
^bSpecific activity: total activity/yield;
^cPurification fold: specific activity of each step/specific activity of the first step.