Research Article

A Phytase Characterized by Relatively High pH Tolerance and Thermostability from the Shiitake Mushroom Lentinus edodes

Table 2

Characteristics of L. edodes phytase with other fungal phytases.

SpeciesAmino acid sequenceChromatography behaviorMolecular mass (kDa)pH optimumTemperature optimum (°C)

L. edodes (this study)1 DPKRTDQVN 9Unadsorbed on DEAE-cellulose, CM-cellulose, blue gel, and Q-Sepharose145.037
Flammulina velutipes [18]1 DFQVDTGNN 10Adsorbed on DEAE-cellulose and Q-Sepharose; unadsorbed on CM-cellulose and Blue gel14.85.045
Volvariella volvacea [19]1 GEDNEHDTQA 10Adsorbed on Q-Sepharose; unadsorbed on DEAE-cellulose, CM-cellulose, and Blue gel145.045
Aspergillus niger (PhyA) [8, 20, 21] (emb: CAA78904.1)218 DSELADTVE 227852.5, 5.058
A. niger (PhyB) [8, 22] (pdb:1QFX)46 DPPTSCEVDQVI 5785–1002.560
A. ficcum (PhyB) [2]46 DPPTSCEVDQVI 57Adsorbed on DEAE-cellulose and CM-cellulose65.51.367
Kodamaea ohmeri [1, 2]
(ABU53001.1)
24 TPEQAAVEQYN 34Adsorbed on DEAE sepharose98.25.065

—: no data available. Sequence analysis using DNAMAN V6.0.3.99.
Amino acid residues identical to corresponding residues of L. edodes phytase are underlined.