BioMed Research International / 2013 / Article / Tab 2 / Research Article
A Phytase Characterized by Relatively High pH Tolerance and Thermostability from the Shiitake Mushroom Lentinus edodes Table 2 Characteristics of L. edodes phytase with other fungal phytases.
Species Amino acid sequence Chromatography behavior Molecular mass (kDa) pH optimum Temperature optimum (°C) L. edodes (this study)1 DPKRTDQVN 9 Unadsorbed on DEAE-cellulose, CM-cellulose, blue gel, and Q-Sepharose 14 5.0 37 Flammulina velutipes [18 ]1 D FQVDTGN N 10 Adsorbed on DEAE-cellulose and Q-Sepharose; unadsorbed on CM-cellulose and Blue gel 14.8 5.0 45 Volvariella volvacea [19 ]1 GEDNEHDTQA 10 Adsorbed on Q-Sepharose; unadsorbed on DEAE-cellulose, CM-cellulose, and Blue gel 14 5.0 45 Aspergillus niger (PhyA) [8 , 20 , 21 ] (emb: CAA78904.1)218 D SELAD TV E 227 — 85 2.5, 5.0 58 A. niger (PhyB) [8 , 22 ] (pdb:1QFX)46 DP PTSCEVDQV I 57 — 85–100 2.5 60 A. ficcum (PhyB) [2 ]46 DP PTSCEVDQV I 57 Adsorbed on DEAE-cellulose and CM-cellulose 65.5 1.3 67 Kodamaea ohmeri [1 , 2 ] (ABU53001.1)24 TP EQAAVEQ YN 34 Adsorbed on DEAE sepharose 98.2 5.0 65
—: no data available. Sequence analysis using DNAMAN V6.0.3.99. Amino acid residues identical to corresponding residues of L. edodes phytase are underlined.