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BioMed Research International
Volume 2013, Article ID 542810, 5 pages
Research Article

Growth Induction and Low-Oxygen Apoptosis Inhibition of Human CD34+ Progenitors in Collagen Gels

1Laboratorio di Biologia Vascolare e Medicina Rigenerativa, Centro Cardiologico Monzino, 20138 Milano, Italy
2Laboratorio di Patologia Vascolare, Istituto Dermopatico dell’Immacolata, 00166 Rome, Italy
3Institute of Biofunctional Polymer Material, Leibniz-Institüt für Polymerforschung, 01069 Dresden, Germany
4Laboratorio di Ingegneria Tissutale Cardiovascolare, Centro Cardiologico Monzino IRCCS, Via Parea 4, 20138 Milano, Italy

Received 26 July 2012; Accepted 8 August 2012

Academic Editor: Maria Elena Padin Iruegas

Copyright © 2013 Daniele Avitabile et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Various reports have indicated low survival of injected progenitors into unfavorable environments such as the ischemic myocardium or lower limb tissues. This represents a major bottleneck in stem-cell-based cardiovascular regenerative medicine. Strategies to enhance survival of these cells in recipient tissues have been therefore sought to improve stem cell survival and ensure long-term engraftment. In the present contribution, we show that embedding human cord blood-derived CD34+ cells into a collagen I-based hydrogel containing cytokines is a suitable strategy to promote stem cell proliferation and protect these cells from anoxia-induced apoptosis.