Research Article

Growth Induction and Low-Oxygen Apoptosis Inhibition of Human CD34+ Progenitors in Collagen Gels

Figure 2

Increased survival of 3D versus 2D cultured CD34+ cells under severe hypoxia conditions. (a-b) Representative flow cytometry contour plots showing the physical appearance (FSC-SSC plot) and the annexinV/PI staining of CD34+ cells cultured for 7 days in an atmosphere containing 95%N2/5%CO2. Multicolor staining of the different regions indicates, respectively, viable cells (green), early apoptotic cells (red), late apoptotic cells (blue), and necrotic cells (gray). (c) Quantification of viable and late apoptosis cells. No significant differences were observed in viable and late apoptotic cells’ number in 2D cultures (±cytokines) and 3D culture without cytokines; by contrast, the number of viable cells was significantly increased and that of late apoptotic cells was significantly reduced, by cytokines-supplemented matrix culture. * indicates by paired Student’s t-test (3D cytokines versus 3D control); .
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