Development of a Generic Microfluidic Device for Simultaneous Detection of Antibodies and Nucleic Acids in Oral Fluids
Unique modular components of the CARD. (A) Saliva collector with removable solid Porex matrix. (B) Illustration of the LF strip compartment and the LF strip schematic: the LF strip contains an extended sample pad that is positioned in a trough that prevents overflow of the LF sample pad and strip. (C) Schematic of the compartment with the NA-binding silica membrane; the inlet/outlet of the connected channels are indicated. (D) Schematic of the amplification microtube indicates how the lumen functions as both inlet and outlet. During amplification the opening of the lumen is well above the liquid surface. After amplification, HSLF buffer is added to tube through the lumen, and as a result the opening of the lumen will be below the liquid surface allowing it to function as an outlet.