BioMed Research International / 2013 / Article / Fig 4

Research Article

Development of a Generic Microfluidic Device for Simultaneous Detection of Antibodies and Nucleic Acids in Oral Fluids

Figure 4

The consecutive flow protocol. (a) Schematic for the analysis of the antibody path, the Test Line was a proprietary peptide mix (OraSure Technologies), and the Flow Line anti-human IgG. (b) Schematic showing consecutive flow applied to detect RT-PCR amplicons provided with a digoxigenin and biotin hapten as described by Corstjens et al. [8], which was developed to detect antibodies against infectious disease pathogens. For analysis of amplicons, the Test line was antidigoxigenin and the Flow Line digoxigenin.
(a)  Antibody (AB) detection 
(b) RNA detection