Photosensitizer Adhered to Cell Culture Microplates Induces Phototoxicity in Carcinoma Cells
Figure 2
Distribution of microplate-adhered PS after addition of cells. The distribution of PS from microplates was determined by plates that were preincubated with 1.0 μM hypericin (a) or 5.0 μM hypericin (b); 1.0 μM PVP-hypericin (c) or 5.0 μM PVP-hypericin (d); 0.4 μM Foscan (e) or 2.0 μM Foscan (f) or 10 μM AlPCS4 (g) and 50 μM AlPCS4 (h). The amount of PS was assessed directly in the incubation plate (MP1) or cells were transferred from the incubation microplate to a new microplate to analyze the PS remaining adhered to the plate (MP2) or having been bound to cells (MP3). Parameters for fluorescence intensity measurements are listed in Table 2. Results are displayed relative to controls preincubated with PS but without cells. Abbreviations: MP: microplate; PS: photosensitizer.
