Intracellular Secretory Leukoprotease Inhibitor Modulates Inositol 1,4,5-Triphosphate Generation and Exerts an Anti-Inflammatory Effect on Neutrophils of Individuals with Cystic Fibrosis and Chronic Obstructive Pulmonary Disease

Figure 5

SLPI inhibits intracellular Ca²⁺ flux. Neutrophils (2 × 10⁵/mL) were isolated from healthy donors ((a) and (b)), individuals with CF ((c) and (d)) or COPD ((e) and (f)) (𝑛=5 for each cohort). Cells remained untreated (control, con) or treated with 480 nM SLPI for 5 min prior to stimulation with fMLP (1 μM) or IL-8 (1.2 nM) for 10, 20, 30, 40, or 50 sec. Intracellular cytosolic Ca²⁺ was analysed using the Invitrogen Fluo-4NW Calcium Assay kit. Each point is the mean ± S.E. (𝑛=5, *𝑃<0.05 and **𝑃<0.001 between untreated and SLPI treated cells calculated by 2 way ANOVA followed by Bonferroni test).

(a)

(b)

(c)

(d)

(e)

(f)