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(a)
560141.fig.005b
(b)
560141.fig.005c
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(f)
Figure 5: SLPI inhibits intracellular Ca2+ flux. Neutrophils (2 × 105/mL) were isolated from healthy donors ((a) and (b)), individuals with CF ((c) and (d)) or COPD ((e) and (f)) ( for each cohort). Cells remained untreated (control, con) or treated with 480 nM SLPI for 5 min prior to stimulation with fMLP (1 μM) or IL-8 (1.2 nM) for 10, 20, 30, 40, or 50 sec. Intracellular cytosolic Ca2+ was analysed using the Invitrogen Fluo-4NW Calcium Assay kit. Each point is the mean ± S.E. ( , * and ** between untreated and SLPI treated cells calculated by 2 way ANOVA followed by Bonferroni test).