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BioMed Research International
Volume 2013, Article ID 560141, 18 pages
Research Article

Intracellular Secretory Leukoprotease Inhibitor Modulates Inositol 1,4,5-Triphosphate Generation and Exerts an Anti-Inflammatory Effect on Neutrophils of Individuals with Cystic Fibrosis and Chronic Obstructive Pulmonary Disease

Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Education & Research Centre, Beaumont Hospital, Dublin 9, Ireland

Received 2 April 2013; Revised 1 July 2013; Accepted 15 July 2013

Academic Editor: Edineia Lemos de Andrade

Copyright © 2013 Emer P. Reeves et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Data: An evaluation of the effect of SLPI compared to calpastatin on calpain activity. The inhibition constant (Ki) of SLPI and calpastatin (endogenous calpain inhibitor) against calpain 1 (Supplementary Figure 1 A and C) and calpain 2 (Supplementary Figure 1 B and D) activity was quantified. GraphPad Prism version 4.03 was employed to calculate Ki values with global curve fit performed finding the single best-fit estimate. Each line represents nonlinear regression analysis of simulated rates of metabolite formation (velocity) as a function of substrate concentration [S]. Results demonstrated the Ki value of calpastatin on calpain 1 and 2 activity as 5.548nM and 1.548nM, respectively (Supplementary Figure 1 A and B). SLPI had no detectable inhibitory effect on calpain 1 and 2 activity resulting in no quantifiable inhibitory constant (Supplementary Figure 1C and E).

  1. Supplementary Material