Figure 4: The TvLEGU-1 protein is localized on the surface and cytoplasm of T. vaginalis. Parasites grown in iron-rich conditions, fixed nonpermeabilized (NP) were incubated with the PI serum, (1 : 100 dilution) as a negative control (a, b, c, and d). Nonpermeabilized (NP; e, f, g, and h) and permeabilized (P; i, j, k, and l) parasites were incubated with the anti-TvLEGU-1r antibody (1 : 100 dilution). Anti-rabbit IgG-FITC (in green) was used as a secondary antibody (1 : 100 dilution) (a, e, and i). Parasite membranes were labeled with DIL (in red; b, f, and j). Nuclei were labeled with DAPI (in blue; c, g, and k). Merge (d, h, and l) in yellow indicates colocalization. Bars: 15 μm (d, h, and l).