Aminopeptidase N (CD13) Is Involved in Phagocytic Processes in Human Dendritic Cells and Macrophages

Figure 4

CD13 localizes at the phagosomal membrane after phagocytosis of zymosan particles in human monocyte-derived macrophages. Human MD-Ms were incubated with Texas Red-labeled zymosan particles for 30 min at 37°C. After washing away noningested particles, cells were fixed, permeabilized, and stained with anti-CD13 antibody and a secondary FITC-labeled goat anti-murine IgG. Samples were analyzed using confocal microscopy. From left to right: images of the same cell taken at 0.75 μm intervals in the 𝑧 axis. Bar: 5.0 μm.