Transcriptomic Profiling of the Four Adenosine Receptors in Human Leukocytes of Heart Failure Patients

Table 2

Details of specific primers used in Real-Time PCR experiments.

Gene	Primer sequence	GenBank, accession number	Amplicon length, bp	Temperature, °C	Efficiency, %	𝑅^2
CD39	F: CACAGCATAGTAGATTGACAT	NM_001776	103	60	99.8	0.998
	R: ATACGCAGACAGAAGGAA
CD73	F: TAAGCACACTGTCTCATT	NM_002526	116	60	101	0.999
	R: CTGTATGGTCAAGTCAAC
YWHAZ	F: ATGCAACCAACACATCCTATC	NM_001135702	178	60	95.3	0.997
	R: GCATTATTAGCGTGCTGTCTT
TOP2b	F: AACTGGATGATGCTAATGATGCT	NM_001068	137	60	98.4	0.998
	R: TGGAAAAACTCCGTATCTGTCTC
SRP14	F: CAGATGGCTTATTCAAACCTCCT	NM_003134	181	60	99.9	0.998
	R: ATGCCCTTTACTGTGCTGCT

CD39: ectonucleoside triphosphate diphosphohydrolase; CD73: ecto-5′-nucleotidase; YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein z polypeptide; TOP2b: topoisomerase II Beta; SRP14: signal recognition particle 14 kDa.