Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates

Table 1

Observed and predicted values of endoglucanase activity for the independent variables SCB and CSL concentrations used in central composite rotational design (CCRD), from the crude enzyme extract of Streptomyces misionensis PESB-25.

Run	SCB (%w/v)/Coded level	CSL (%w/v)/Coded level	Endoglucanase activity (U·mL^−1) Observed*	Endoglucanase activity (U·mL^−1) Predicted
1	0.65 (−1)	0.77 (−1)	0.95 ± 0.11	0.91
2	1.35 (+1)	0.77 (−1)	0.72 ± 0.005	0.67
3	0.65 (−1)	1.63 (+1)	0.86 ± 0.003	0.85
4	1.35 (+1)	1.63 (+1)	0.98 ± 0.038	0.96
5	0.5 (−1.41)	1.2 (0)	0.93 ± 0.022	0.95
6	1.5 (1.41)	1.2 (0)	0.83 ± 0.032	0.86
7	1.0 (0)	0.6 (−1.41)	0.66 ± 0.003	0.71
8	1.0 (0)	1.8 (1.41)	0.87 ± 0.024	0.88
9	1.0 (0)	1.2 (0)	1.03 ± 0.016	1.01
10	1.0 (0)	1.2 (0)	1.03 ± 0.044	1.01
11	1.0 (0)	1.2 (0)	1.00 ± 0.003	1.01

The statistical analysis of the results was performed using the software Design Expert 7.0 (trial version).
*Values are based on Mean ± SD of 3 individual observations.