Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates

Table 3

Observed and predicted values of endoglucanase activity for the independent variables pH and temperature used in CCRD, from the crude enzyme preparation of Streptomyces misionensis PESB-25.

Run	pH/Coded level	Temperature (°C)/Coded level	Endoglucanase activity; (U·mL^−1); Observed	Endoglucanase activity; (U·mL^−1); Predicted
1	3.6 (−1)	44 (−1)	1.02	0.96
2	6.4 (+1)	44 (−1)	0.77	0.78
3	3.6 (−1)	66 (+1)	1.53	1.42
4	6.4 (+1)	66 (+1)	0.81	0.77
5	3.0 (−1.41)	55 (0)	1.21	1.31
6	7.0 (1.41)	55 (0)	0.72	0.72
7	5.0 (0)	40 (−1.41)	0.78	0.79
8	5.0 (0)	70 (1.41)	1.02	1.11
9	5.0 (0)	55 (0)	1.27	1.23
10	5.0 (0)	55 (0)	1.26	1.23
11	5.0 (0)	55 (0)	1.18	1.23
12	5.0 (0)	55 (0)	1.20	1.23